Hotspot

trnQ-UUG-psbK

LSC · 8085-9203 · 1119 bp

Back to Cyanotis

Pi 0.0901
MarkerSeek score 76.80
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
1119
variable_sites
71
pi
0.0901
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.70
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GACCAAAACCCGTTGCCTTA TCGAATAAAGTACAGAATTAAGACAGA 50.0 29.6 58.7 56.6 936-1212 bp 1.0 49.3
trnQ-UUG-psbK_p2 GACCAAAACCCGTTGCCTTA CGAATAAAGTACAGAATTAAGACAGA 50.0 30.8 58.7 55.4 935-1211 bp 1.0 49.3
trnQ-UUG-psbK_p3 GACCAAAACCCGTTGCCTTA TCGAATAAAGTACAGAATTAAGACAG 50.0 30.8 58.7 55.4 936-1212 bp 1.0 49.3
trnQ-UUG-psbK_p4 ACCAAAACCCGTTGCCTTAC TCGAATAAAGTACAGAATTAAGACAGA 50.0 29.6 59.0 56.6 935-1211 bp 1.0 49.3
trnQ-UUG-psbK_p5 GACCAAAACCCGTTGCCTTA TCGAATAAAGTACAGAATTAAGACA 50.0 28.0 58.7 54.4 936-1212 bp 1.0 49.3