Hotspot

rps16-trnQ-UUG

LSC · 7006-8012 · 1007 bp

Back to Cyanotis

Pi 0.044
MarkerSeek score 78.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1007
variable_sites
39
pi
0.044
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1098-2053 bp 1.0 85.1
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1096-2051 bp 1.0 85.1
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1104-2059 bp 1.0 85.0
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1105-2060 bp 1.0 85.0
rps16-trnQ-UUG_p5 AGAATCCACAGCCGATCATG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1106-2061 bp 1.0 83.4