Hotspot

matK-trnK-UUU

LSC · 4020-5294 · 1275 bp

Back to Cyanotis

Pi 0.0543

MarkerSeek score 76.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 62
pi: 0.0543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCGTGAAGAAAGAACCGCAA	GACAATGGTGTGTCGATCGA	45.0	50.0	58.1	58.0	2009-2061 bp	1.0	86.9
matK-trnK-UUU_p2	TTCGTGAAGAAAGAACCGCA	GACAATGGTGTGTCGATCGA	45.0	50.0	58.1	58.0	2010-2062 bp	1.0	86.9
matK-trnK-UUU_p3	AAGAGACTTCGTGGATCCGA	GACAATGGTGTGTCGATCGA	50.0	50.0	58.2	58.0	1978-2030 bp	1.0	86.0
matK-trnK-UUU_p4	AGAGACTTCGTGGATCCGAA	GACAATGGTGTGTCGATCGA	50.0	50.0	58.2	58.0	1977-2029 bp	1.0	86.0
matK-trnK-UUU_p5	TGAAATTTCCCCAAGACCCG	GACAATGGTGTGTCGATCGA	50.0	50.0	58.1	58.0	1831 bp	0.5	59.3