Hotspot

ycf27

SSC · 38276-38995 · 720 bp

Back to Cyanophora

Pi 0.432

MarkerSeek score 80.50

Primer available yes

Label ycf27

Feature Metadata

label_name: ycf27
feature_type: gene
region: SSC
length_bp: 720
variable_sites: 261
pi: 0.432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf27_p1	TTGGAGCTAAAAAGTGGCGT	ATTCTCACGCGTTACTCACC	45.0	50.0	58.0	58.0	1411 bp	0.5	59.7
ycf27_p2	TTGGAGCTAAAAAGTGGCGT	CATTGCGGAAAATTCCCCAC	45.0	50.0	58.0	58.0	1658 bp	0.5	59.7
ycf27_p3	TTGGAGCTAAAAAGTGGCGT	TTTACAACCCACAGGCCTTC	45.0	50.0	58.0	58.0	1714 bp	0.5	59.7
ycf27_p4	TTGGAGCTAAAAAGTGGCGT	AGCTACTGATCGTTGCCTTG	45.0	50.0	58.0	58.0	1573 bp	0.5	59.7
ycf27_p5	TTGGAGCTAAAAAGTGGCGT	CAGCTACTGATCGTTGCCTT	45.0	50.0	58.0	58.0	1574 bp	0.5	59.7