Hotspot

ycf21

SSC · 34714-35322 · 609 bp

Back to Cyanophora

Pi 0.423

MarkerSeek score 82.80

Primer available yes

Label ycf21

Feature Metadata

label_name: ycf21
feature_type: gene
region: SSC
length_bp: 609
variable_sites: 238
pi: 0.423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf21_p1	AACTTTACTACCCGTGCGTC	GGAGAAGCAAATCCACCACA	50.0	50.0	57.9	58.1	1475 bp	0.5	58.7
ycf21_p2	CTTTACTACCCGTGCGTCTC	GGAGAAGCAAATCCACCACA	55.0	50.0	58.1	58.1	1473 bp	0.5	58.5
ycf21_p3	AACTTTACTACCCGTGCGTC	ATGGGTTCTCGCCTTTTCTC	50.0	50.0	57.9	57.9	1581 bp	0.5	58.5
ycf21_p4	AACTTTACTACCCGTGCGTC	GATGGGTTCTCGCCTTTTCT	50.0	50.0	57.9	57.9	1582 bp	0.5	58.5
ycf21_p5	GCTCGTCTTCCACTACGATC	GGAGAAGCAAATCCACCACA	55.0	50.0	57.9	58.1	1750 bp	0.5	58.4