Hotspot

rbcR

SSC · 33395-34369 · 975 bp

Back to Cyanophora

Pi 0.429

MarkerSeek score 81.90

Primer available yes

Label rbcR

Feature Metadata

label_name: rbcR
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 405
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR_p1	TTAGTAGGTTTTCCGCGTCG	GACGCACGGGTAGTAAAGTT	50.0	50.0	58.0	57.9	1449 bp	0.5	59.3
rbcR_p2	TTAGTAGGTTTTCCGCGTCG	GAGACGCACGGGTAGTAAAG	50.0	55.0	58.0	58.1	1451 bp	0.5	59.1
rbcR_p3	AAGCCATTTCCATGCTTCCA	GACGCACGGGTAGTAAAGTT	45.0	50.0	58.1	57.9	1346 bp	0.5	59.0
rbcR_p4	CCATGCTTCCACCAGCTAAT	GACGCACGGGTAGTAAAGTT	50.0	50.0	57.9	57.9	1337 bp	0.5	58.9
rbcR_p5	AAGCCATTTCCATGCTTCCA	GAGACGCACGGGTAGTAAAG	45.0	55.0	58.1	58.1	1348 bp	0.5	58.8