Hotspot

psaC

SSC · 25580-25825 · 246 bp

Back to Cyanophora

Pi 0.525

MarkerSeek score 73.30

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 104
pi: 0.525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AGCCAACGCAATTGTATTGC	TTCAATCAAATCGGCACCCA	45.0	45.0	58.0	58.1	1264 bp	0.5	59.3
psaC_p2	GCCAACGCAATTGTATTGCT	TTCAATCAAATCGGCACCCA	45.0	45.0	58.0	58.1	1263 bp	0.5	59.3
psaC_p3	TGGTCGTAGGTTCAAGTCCT	TTCAATCAAATCGGCACCCA	50.0	45.0	58.0	58.1	946 bp	0.5	59.3
psaC_p4	TTCAAGTCCTACCTGGGGAG	TTCAATCAAATCGGCACCCA	55.0	45.0	58.0	58.1	936 bp	0.5	59.0
psaC_p5	GCCAACGCAATTGTATTGCT	TCAATCAAATCGGCACCCAT	45.0	45.0	58.0	57.9	1262 bp	0.5	58.8