Hotspot

mntA

SSC · 27688-28422 · 735 bp

Back to Cyanophora

Pi 0.475

MarkerSeek score 83.50

Primer available yes

Label mntA

Feature Metadata

label_name: mntA
feature_type: gene
region: SSC
length_bp: 735
variable_sites: 329
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
mntA_p1	TGCAATTGCTAAACCCGGTA	GGATGAGAATTATCGTTGGCC	45.0	47.6	57.8	56.8	1047 bp	0.5	41.0
mntA_p2	TGCAATTGCTAAACCCGGTA	TGAGAATTATCGTTGGCCTTCA	45.0	40.9	57.8	57.8	1044 bp	0.5	40.7
mntA_p3	TGCAATTGCTAAACCCGGTA	AGGATGAGAATTATCGTTGGCC	45.0	45.5	57.8	58.3	1048 bp	0.5	40.3
mntA_p4	TGCAATTGCTAAACCCGGTA	GGATGAGAATTATCGTTGGCCT	45.0	45.5	57.8	58.3	1047 bp	0.5	40.3
mntA_p5	TAAACCAGTTGCACCAGGTG	GGATGAGAATTATCGTTGGCC	50.0	47.6	58.3	56.8	1515 bp	0.5	40.1