Hotspot

ftsW

SSC · 23857-25047 · 1191 bp

Back to Cyanophora

Pi 0.438

MarkerSeek score 76.70

Primer available yes

Label ftsW

Feature Metadata

label_name: ftsW
feature_type: gene
region: SSC
length_bp: 1191
variable_sites: 368
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsW_p1	AACCGGCTGTAATCCATCTA	AGGACTTGAACCTACGACCA	45.0	50.0	56.0	58.0	2130 bp	0.5	44.1
ftsW_p2	ACCGGCTGTAATCCATCTAA	AGGACTTGAACCTACGACCA	45.0	50.0	56.0	58.0	2129 bp	0.5	44.1
ftsW_p3	TAACCGGCTGTAATCCATCT	AGGACTTGAACCTACGACCA	45.0	50.0	56.0	58.0	2131 bp	0.5	44.1
ftsW_p4	AACCGGCTGTAATCCATCTA	CTCCCCAGGTAGGACTTGAA	45.0	55.0	56.0	58.0	2140 bp	0.5	43.7
ftsW_p5	ACCGGCTGTAATCCATCTAA	CTCCCCAGGTAGGACTTGAA	45.0	55.0	56.0	58.0	2139 bp	0.5	43.7