Hotspot

ftsQ

SSC · 28481-29335 · 855 bp

Back to Cyanophora

Pi 0.425

MarkerSeek score 82.70

Primer available yes

Label ftsQ

Feature Metadata

label_name: ftsQ
feature_type: gene
region: SSC
length_bp: 855
variable_sites: 334
pi: 0.425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsQ_p1	TGTCCACCAGAAAGTTCACG	GCATCTGCGCGTATTGAATC	50.0	50.0	58.1	58.1	1885 bp	0.5	59.1
ftsQ_p2	TTTCCTGCTCCATTTGGTCC	GCATCTGCGCGTATTGAATC	50.0	50.0	58.1	58.1	1588 bp	0.5	58.9
ftsQ_p3	TCCTGAGCAAGTAAACGAGC	GCATCTGCGCGTATTGAATC	50.0	50.0	57.9	58.1	1924 bp	0.5	58.9
ftsQ_p4	CCTGAGCAAGTAAACGAGCT	GCATCTGCGCGTATTGAATC	50.0	50.0	57.9	58.1	1923 bp	0.5	58.9
ftsQ_p5	CCTGCTCCATTTGGTCCAAT	GCATCTGCGCGTATTGAATC	50.0	50.0	58.1	58.1	1585 bp	0.5	58.4