Hotspot

chlN

SSC · 36427-37803 · 1377 bp

Back to Cyanophora

Pi 0.455

MarkerSeek score 83.10

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: SSC
length_bp: 1377
variable_sites: 582
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	TGTGGTGGATTTGCTTCTCC	ACGCCACTTTTTAGCTCCAA	50.0	45.0	58.1	58.0	2082 bp	0.5	59.1
chlN_p2	TGTGGTGGATTTGCTTCTCC	GGAGAAGCTTTTTCACGTGC	50.0	50.0	58.1	58.0	2553 bp	0.5	59.0
chlN_p3	AGAAAAGGCGAGAACCCATC	ACGCCACTTTTTAGCTCCAA	50.0	45.0	57.9	58.0	1975 bp	0.5	58.9
chlN_p4	GAGAAAAGGCGAGAACCCAT	ACGCCACTTTTTAGCTCCAA	50.0	45.0	57.9	58.0	1976 bp	0.5	58.9
chlN_p5	TGTGGTGGATTTGCTTCTCC	AAACGTGGTCATCGACAACA	50.0	45.0	58.1	58.1	2018 bp	0.5	58.8