Hotspot

chlL

SSC · 35531-36382 · 852 bp

Back to Cyanophora

Pi 0.472

MarkerSeek score 81.50

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: SSC
length_bp: 852
variable_sites: 351
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	TAACGACCCCAAAAAGGACC	TCGGGGTTCAGCAAAAATCA	50.0	45.0	57.7	58.0	1799 bp	0.5	57.7
chlL_p2	TAACGACCCCAAAAAGGACC	GCGTAGTCCAAACCATTTGC	50.0	50.0	57.7	58.0	2032 bp	0.5	57.6
chlL_p3	CGACCCCAAAAAGGACCTTT	TCGGGGTTCAGCAAAAATCA	50.0	45.0	58.3	58.0	1796 bp	0.5	57.5
chlL_p4	CGACCCCAAAAAGGACCTTT	GCGTAGTCCAAACCATTTGC	50.0	50.0	58.3	58.0	2029 bp	0.5	57.4
chlL_p5	TAACGACCCCAAAAAGGACC	GTCCAAACCATTTGCACGAG	50.0	50.0	57.7	57.9	2027 bp	0.5	57.4