Hotspot

ccsA

SSC · 22358-23317 · 960 bp

Back to Cyanophora

Pi 0.429

MarkerSeek score 77.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 297
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGTTTTGGACGGCTTTTACG	ACAGGTTTACCTTTGCCGTT	50.0	45.0	58.0	57.9	2064 bp	0.5	59.2
ccsA_p2	AGGTGGTGGGGTTCGTAATA	ACAGGTTTACCTTTGCCGTT	50.0	45.0	58.0	57.9	2044 bp	0.5	59.2
ccsA_p3	GGTTTTGGACGGCTTTTACG	GAATTTTCCCGACCACAGGT	50.0	50.0	58.0	58.1	2078 bp	0.5	58.9
ccsA_p4	AGGTGGTGGGGTTCGTAATA	GAATTTTCCCGACCACAGGT	50.0	50.0	58.0	58.1	2058 bp	0.5	58.9
ccsA_p5	GCTTGGACAGAAGATGGGTT	ACAGGTTTACCTTTGCCGTT	50.0	45.0	57.8	57.9	2325 bp	0.5	57.9