Hotspot

trnL-trnE

LSC · 50342-50471 · 130 bp

Back to Cyanophora

Pi 0.195

MarkerSeek score 81.20

Primer available yes

Label trnL-trnE

Feature Metadata

label_name: trnL-trnE
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 24
pi: 0.195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnE_p1	TTGCGTCTACCAATTCCACC	CCTAGGACACCTCCCTTTCA	50.0	55.0	58.2	58.0	207-214 bp	1.0	88.8
trnL-trnE_p2	TTGCGTCTACCAATTCCACC	CTATGCCCCCATCGTCTAGA	50.0	55.0	58.2	58.1	229-236 bp	1.0	88.3
trnL-trnE_p3	TTGCGTCTACCAATTCCACC	TATGCCCCCATCGTCTAGAG	50.0	55.0	58.2	58.1	228-235 bp	1.0	88.3
trnL-trnE_p4	TCCGTTGCGTCTACCAATTC	CCTAGGACACCTCCCTTTCA	50.0	55.0	58.3	58.0	211-218 bp	1.0	88.0
trnL-trnE_p5	TCCGTTGCGTCTACCAATTC	TATGCCCCCATCGTCTAGAG	50.0	55.0	58.3	58.1	232-239 bp	1.0	87.5