Hotspot

ORF243

LSC · 11708-12439 · 732 bp

Pi 0.247

MarkerSeek score 80.40

Primer available yes

Label ORF243

Feature Metadata

label_name: ORF243
feature_type: gene
region: LSC
length_bp: 732
variable_sites: 151
pi: 0.247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF243_p1	AGCGAACATCAATTGCAACG	CAAAGATTGCTGCGGGAAAG	45.0	50.0	58.0	58.0	2014-2294 bp	1.0	91.6
ORF243_p2	GCGAACATCAATTGCAACGA	CAAAGATTGCTGCGGGAAAG	45.0	50.0	58.0	58.0	2013-2293 bp	1.0	91.6
ORF243_p3	TGCTTTCTACCACAGCGTTT	CAAAGATTGCTGCGGGAAAG	45.0	50.0	58.0	58.0	1976-2256 bp	1.0	91.6
ORF243_p4	TTGCTTTCTACCACAGCGTT	CAAAGATTGCTGCGGGAAAG	45.0	50.0	58.0	58.0	1977-2257 bp	1.0	91.6
ORF243_p5	AGCGAACATCAATTGCAACG	GGCAAATCGTCCCAATTTCG	45.0	50.0	58.0	58.1	1242 bp	0.5	59.0