Hotspot

ORF225

LSC · 57358-58035 · 678 bp

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Pi 0.103
MarkerSeek score 78.90
Primer available yes
Label ORF225

Feature Metadata

label_name
ORF225
feature_type
gene
region
LSC
length_bp
678
variable_sites
70
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF225_p1 CTAGAAAGTTTCTCCGGCCC TCGACAGAAGCTGCTTCATC 55.0 50.0 58.0 58.0 1541 bp 0.5 59.8
ORF225_p2 CTAGAAAGTTTCTCCGGCCC CGACAGAAGCTGCTTCATCT 55.0 50.0 58.0 58.0 1540 bp 0.5 59.7
ORF225_p3 AAAGCAGAAGCATAGACGCA TCGACAGAAGCTGCTTCATC 45.0 50.0 57.9 58.0 1426 bp 0.5 59.2
ORF225_p4 AAACAACAAAGGAGGCCCAT TCGACAGAAGCTGCTTCATC 45.0 50.0 57.9 58.0 997 bp 0.5 59.2
ORF225_p5 AAAGCAGAAGCATAGACGCA CGACAGAAGCTGCTTCATCT 45.0 50.0 57.9 58.0 1425 bp 0.5 59.1