Hotspot

trnI (CAU)-rpl23

IRa · 162475-163225 · 751 bp

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Pi 0.106
MarkerSeek score 76.10
Primer available yes
Label trnI (CAU)-rpl23

Feature Metadata

label_name
trnI (CAU)-rpl23
feature_type
igs
region
IRa
length_bp
751
variable_sites
41
pi
0.106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.51
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI (CAU)-rpl23_p1 CCTGTTAAGCATCCATGGCT TTTCCCCAATAACCGACGAC 50.0 50.0 57.9 57.9 535-890 bp 1.0 87.3
trnI (CAU)-rpl23_p2 CCTGTTAAGCATCCATGGCT CCCCAATAACCGACGACTTT 50.0 50.0 57.9 57.9 532-887 bp 1.0 87.3
trnI (CAU)-rpl23_p3 CCTGTTAAGCATCCATGGCT AACCGACGACTTTTGTCTGT 50.0 45.0 57.9 57.7 525-880 bp 1.0 85.7
trnI (CAU)-rpl23_p4 ACGAGGATCCCTGTTAAGCA TTTCCCCAATAACCGACGAC 50.0 50.0 58.4 57.9 544-899 bp 1.0 84.3
trnI (CAU)-rpl23_p5 ACGAGGATCCCTGTTAAGCA CCCCAATAACCGACGACTTT 50.0 50.0 58.4 57.9 541-896 bp 1.0 84.3