Hotspot

rpl23-trnI (CAU)

IRb · 84607-85357 · 751 bp

Back to Cyanea

Pi 0.106
MarkerSeek score 76.10
Primer available yes
Label rpl23-trnI (CAU)

Feature Metadata

label_name
rpl23-trnI (CAU)
feature_type
igs
region
IRb
length_bp
751
variable_sites
41
pi
0.106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.51
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI (CAU)_p1 TTTCCCCAATAACCGACGAC CCTGTTAAGCATCCATGGCT 50.0 50.0 57.9 57.9 535-890 bp 1.0 87.3
rpl23-trnI (CAU)_p2 CCCCAATAACCGACGACTTT CCTGTTAAGCATCCATGGCT 50.0 50.0 57.9 57.9 532-887 bp 1.0 87.3
rpl23-trnI (CAU)_p3 AACCGACGACTTTTGTCTGT CCTGTTAAGCATCCATGGCT 45.0 50.0 57.7 57.9 525-880 bp 1.0 85.7
rpl23-trnI (CAU)_p4 TTTCCCCAATAACCGACGAC ACGAGGATCCCTGTTAAGCA 50.0 50.0 57.9 58.4 544-899 bp 1.0 84.3
rpl23-trnI (CAU)_p5 CCCCAATAACCGACGACTTT ACGAGGATCCCTGTTAAGCA 50.0 50.0 57.9 58.4 541-896 bp 1.0 84.3