Hotspot

rpl16-rps3

LSC · 81116-81280 · 165 bp

Back to Cyanea

Pi 0.0494
MarkerSeek score 72.00
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
165
variable_sites
8
pi
0.0494
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AAACCAACGAGTCACACACT CGAACTATTTCTGGGATATTAGGC 45.0 41.7 57.6 57.0 248-249 bp 1.0 43.5
rpl16-rps3_p2 AAACCAACGAGTCACACACT TCGAACTATTTCTGGGATATTAGGC 45.0 40.0 57.6 58.3 249-250 bp 1.0 43.5
rpl16-rps3_p3 TGCGAAGTGATGAATTGGGT CGAACTATTTCTGGGATATTAGGC 45.0 41.7 57.8 57.0 323-325 bp 1.0 42.7
rpl16-rps3_p4 TGCGAAGTGATGAATTGGGT TCGAACTATTTCTGGGATATTAGGC 45.0 40.0 57.8 58.3 324-326 bp 1.0 42.7
rpl16-rps3_p5 TGCGAAGTGATGAATTGGGT CGAACTATTTCTGGGATATTAGGCA 45.0 40.0 57.8 58.5 323-325 bp 1.0 42.7