Hotspot

ndhC-ORF225

LSC · 56919-57357 · 439 bp

Back to Cyanea

Pi 0.0971
MarkerSeek score 76.80
Primer available yes
Label ndhC-ORF225

Feature Metadata

label_name
ndhC-ORF225
feature_type
igs
region
LSC
length_bp
439
variable_sites
37
pi
0.0971
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-ORF225_p1 CTAGAAAGTTTCTCCGGCCC CTCGGGCAAAGTCGAATACA 55.0 50.0 58.0 58.0 969-1027 bp 1.0 91.1
ndhC-ORF225_p2 CTAGAAAGTTTCTCCGGCCC CACTCGGGCAAAGTCGAATA 55.0 50.0 58.0 58.0 971-1029 bp 1.0 91.0
ndhC-ORF225_p3 CTAGAAAGTTTCTCCGGCCC ACTCGGGCAAAGTCGAATAC 55.0 50.0 58.0 58.0 970-1028 bp 1.0 91.0
ndhC-ORF225_p4 TCTCACTAAAGAAGGGGCCA CACTCGGGCAAAGTCGAATA 50.0 50.0 58.0 58.0 1529-1587 bp 1.0 87.5
ndhC-ORF225_p5 TCTCACTAAAGAAGGGGCCA CTCGGGCAAAGTCGAATACA 50.0 50.0 58.0 58.0 1527-1585 bp 1.0 87.5