Hotspot

rpoC1-rpoB

LSC · 29589-29631 · 43 bp

Back to Cyanea

Pi 0.0698
MarkerSeek score 73.10
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
LSC
length_bp
43
variable_sites
3
pi
0.0698
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 TGTTGCGGAGAAACTAATCCA TTGGCTCTGGAACTGAATCA 42.9 45.0 57.6 56.7 168 bp 1.0 60.1
rpoC1-rpoB_p2 TGTTGCGGAGAAACTAATCCA TCTTTGGCTCTGGAACTGAA 42.9 45.0 57.6 56.7 171 bp 1.0 59.5
rpoC1-rpoB_p3 TGTTGCGGAGAAACTAATCCA TGGCTCTGGAACTGAATCAT 42.9 45.0 57.6 56.5 167 bp 1.0 58.3
rpoC1-rpoB_p4 TGTTGCGGAGAAACTAATCCA TTTGGCTCTGGAACTGAATCA 42.9 42.9 57.6 57.5 169 bp 1.0 58.1
rpoC1-rpoB_p5 TGTTGCGGAGAAACTAATCCA TGGCTCTGGAACTGAATCATT 42.9 42.9 57.6 57.3 167 bp 1.0 56.3