Hotspot

rpoC2-rpoC1

LSC · 26616-26781 · 166 bp

Back to Cyanea

Pi 0.0482
MarkerSeek score 80.20
Primer available yes
Label rpoC2-rpoC1

Feature Metadata

label_name
rpoC2-rpoC1
feature_type
igs
region
LSC
length_bp
166
variable_sites
8
pi
0.0482
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rpoC1_p1 TGTGAAAGACCAGATTGGCC GCGAAATCGAAGAGGCCATA 50.0 50.0 58.1 58.1 253 bp 1.0 80.7
rpoC2-rpoC1_p2 TGTGAAAGACCAGATTGGCC GAGGCCATACAGGGGTTTTC 50.0 55.0 58.1 58.2 242 bp 1.0 80.0
rpoC2-rpoC1_p3 TGTGAAAGACCAGATTGGCC TATCGCGAAATCGAAGAGGC 50.0 50.0 58.1 58.2 257 bp 1.0 79.9
rpoC2-rpoC1_p4 TGTGAAAGACCAGATTGGCC TTTCCCAGGCCTGTTCCTAT 50.0 50.0 58.1 58.3 226 bp 1.0 79.6
rpoC2-rpoC1_p5 TGTGAAAGACCAGATTGGCC TTTTCCCAGGCCTGTTCCTA 50.0 50.0 58.1 58.5 227 bp 1.0 77.7