Hotspot

ORF243-trnQ (UUG)

LSC · 12440-12775 · 336 bp

Back to Cyanea

Pi 0.473
MarkerSeek score 73.80
Primer available yes
Label ORF243-trnQ (UUG)

Feature Metadata

label_name
ORF243-trnQ (UUG)
feature_type
igs
region
LSC
length_bp
336
variable_sites
125
pi
0.473
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF243-trnQ (UUG)_p1 AGCGAACATCAATTGCAACG CAAAGATTGCTGCGGGAAAG 45.0 50.0 58.0 58.0 2014-2294 bp 1.0 91.6
ORF243-trnQ (UUG)_p2 GCGAACATCAATTGCAACGA CAAAGATTGCTGCGGGAAAG 45.0 50.0 58.0 58.0 2013-2293 bp 1.0 91.6
ORF243-trnQ (UUG)_p3 TGCTTTCTACCACAGCGTTT CAAAGATTGCTGCGGGAAAG 45.0 50.0 58.0 58.0 1976-2256 bp 1.0 91.6
ORF243-trnQ (UUG)_p4 TTGCTTTCTACCACAGCGTT CAAAGATTGCTGCGGGAAAG 45.0 50.0 58.0 58.0 1977-2257 bp 1.0 91.6
ORF243-trnQ (UUG)_p5 TTTAGATTCGCTTGGAGGGC CAAAGATTGCTGCGGGAAAG 50.0 50.0 58.0 58.0 1944 bp 0.5 59.6