Hotspot

ycf1

IRb · 106727-114079 · 7353 bp

Back to Cyananthus

Pi 0.023

MarkerSeek score 52.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 7353
variable_sites: 166
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGCCTGCAGGAAAGTTTCA	GGGTTTGGTGTGGATGAGTT	45.0	50.0	58.0	58.0	864-966 bp	1.0	84.2
ycf1_p2	ATGCCTGCAGGAAAGTTTCA	AGTCCCCAGAAAGCCCTTAT	45.0	50.0	58.0	58.0	1283-1385 bp	1.0	83.4
ycf1_p3	ATGCCTGCAGGAAAGTTTCA	ATAGTCCCCAGAAAGCCCTT	45.0	50.0	58.0	58.0	1285-1387 bp	1.0	83.4
ycf1_p4	ATGCCTGCAGGAAAGTTTCA	AAGGAAGGGTCGTGTAGGAA	45.0	50.0	58.0	58.0	1325-1427 bp	1.0	83.2
ycf1_p5	ATGCCTGCAGGAAAGTTTCA	AGGAAGGGTCGTGTAGGAAA	45.0	50.0	58.0	58.0	1324-1426 bp	1.0	83.2