Hotspot

trnQ(UUG)

LSC · 54466-54538 · 73 bp

Back to Cyananthus

Pi 0.507

MarkerSeek score 76.40

Primer available yes

Label trnQ(UUG)

Feature Metadata

label_name: trnQ(UUG)
feature_type: tRNA
region: LSC
length_bp: 73
variable_sites: 34
pi: 0.507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ(UUG)_p1	AAAGAAATTCCCCGCGCTAT	CTATTATCGCGCGGAACTGA	45.0	50.0	57.9	58.0	888-1053 bp	1.0	85.5
trnQ(UUG)_p2	AAAGAAATTCCCCGCGCTAT	TCTATTATCGCGCGGAACTG	45.0	50.0	57.9	58.0	889-1054 bp	1.0	85.5
trnQ(UUG)_p3	AAAGAAATTCCCCGCGCTAT	CTAGAGTCAGGCCAAGACCT	45.0	55.0	57.9	58.1	814-979 bp	1.0	85.2
trnQ(UUG)_p4	AAAGAAATTCCCCGCGCTAT	TCTAGAGTCAGGCCAAGACC	45.0	55.0	57.9	58.1	815-980 bp	1.0	85.2
trnQ(UUG)_p5	CGAAAACTTACAGCGGCTTG	AGCAACGCATTTTGAATCCG	50.0	45.0	58.0	58.0	1795-1960 bp	1.0	82.9