Hotspot

ycf1-trnN(GUU)

IRa · 141451-142147 · 697 bp

Back to Cyananthus

Pi 0.0409

MarkerSeek score 69.00

Primer available yes

Label ycf1-trnN(GUU)

Feature Metadata

label_name: ycf1-trnN(GUU)
feature_type: igs
region: IRa
length_bp: 697
variable_sites: 28
pi: 0.0409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN(GUU)_p1	TCCGTATCCAGGCGACTAAT	AAACAAAGACGGGAATGGCT	50.0	45.0	58.0	58.0	1392-2217 bp	1.0	82.5
ycf1-trnN(GUU)_p2	TCCGTATCCAGGCGACTAAT	AACAAAGACGGGAATGGCTT	50.0	45.0	58.0	58.0	1391-2216 bp	1.0	82.5
ycf1-trnN(GUU)_p3	ACCAACAAAACTGCTCGCTA	AAACAAAGACGGGAATGGCT	45.0	45.0	58.0	58.0	1331-2156 bp	1.0	82.3
ycf1-trnN(GUU)_p4	ACCAACAAAACTGCTCGCTA	AACAAAGACGGGAATGGCTT	45.0	45.0	58.0	58.0	1330-2155 bp	1.0	82.3
ycf1-trnN(GUU)_p5	TACTGCTACCCTCTTCTCGG	AACAAAGACGGGAATGGCTT	55.0	45.0	57.9	58.0	1021-1846 bp	1.0	82.2