Hotspot

ndhD-ccsA

SSC · 122325-122561 · 237 bp

Back to Cyananthus

Pi 0.0295

MarkerSeek score 69.50

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 237
variable_sites: 7
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	CCTGTAATAGGCGTTGGGAT	CTGCAATTGTGGCGACTCTA	50.0	50.0	57.1	58.3	449 bp	1.0	71.6
ndhD-ccsA_p2	CCTGTAATAGGCGTTGGGAT	TGCAATTGTGGCGACTCTAG	50.0	50.0	57.1	58.3	448 bp	1.0	71.6
ndhD-ccsA_p3	CCTGTAATAGGCGTTGGGAT	AATTCTGCAATTGTGGCGAC	50.0	45.0	57.1	57.6	453 bp	1.0	71.0
ndhD-ccsA_p4	CCTGTAATAGGCGTTGGGAT	ATTCTGCAATTGTGGCGACT	50.0	45.0	57.1	58.5	452 bp	1.0	70.1
ndhD-ccsA_p5	ATAGGCGTTGGGATGTATCC	TGCAATTGTGGCGACTCTAG	50.0	50.0	56.8	58.3	442 bp	1.0	69.9