Hotspot

trnN(GUU)-ycf1

IRb · 106030-106726 · 697 bp

Back to Cyananthus

Pi 0.0409

MarkerSeek score 69.00

Primer available yes

Label trnN(GUU)-ycf1

Feature Metadata

label_name: trnN(GUU)-ycf1
feature_type: igs
region: IRb
length_bp: 697
variable_sites: 28
pi: 0.0409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN(GUU)-ycf1_p1	AAACAAAGACGGGAATGGCT	TCCGTATCCAGGCGACTAAT	45.0	50.0	58.0	58.0	1392-2217 bp	1.0	82.5
trnN(GUU)-ycf1_p2	AACAAAGACGGGAATGGCTT	TCCGTATCCAGGCGACTAAT	45.0	50.0	58.0	58.0	1391-2216 bp	1.0	82.5
trnN(GUU)-ycf1_p3	AAACAAAGACGGGAATGGCT	ACCAACAAAACTGCTCGCTA	45.0	45.0	58.0	58.0	1331-2156 bp	1.0	82.3
trnN(GUU)-ycf1_p4	AACAAAGACGGGAATGGCTT	ACCAACAAAACTGCTCGCTA	45.0	45.0	58.0	58.0	1330-2155 bp	1.0	82.3
trnN(GUU)-ycf1_p5	AACAAAGACGGGAATGGCTT	TACTGCTACCCTCTTCTCGG	45.0	55.0	58.0	57.9	1021-1846 bp	1.0	82.2