Hotspot

trnR(UCU)-trnG(UCC)

LSC · 51284-51450 · 167 bp

Back to Cyananthus

Pi 0.018

MarkerSeek score 61.90

Primer available yes

Label trnR(UCU)-trnG(UCC)

Feature Metadata

label_name: trnR(UCU)-trnG(UCC)
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 3
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(UCU)-trnG(UCC)_p1	AGGGAAAAATTGCGTCCAAT	AGCCTTCCAAGCTAACGATG	40.0	50.0	56.2	58.0	293 bp	1.0	66.1
trnR(UCU)-trnG(UCC)_p2	AGGGAAAAATTGCGTCCAAT	CCTAGCCTTCCAAGCTAACG	40.0	55.0	56.2	58.1	296 bp	1.0	65.8
trnR(UCU)-trnG(UCC)_p3	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	247 bp	1.0	64.3
trnR(UCU)-trnG(UCC)_p4	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	250 bp	1.0	64.1
trnR(UCU)-trnG(UCC)_p5	AGGGAAAAATTGCGTCCAAT	CCCTAGCCTTCCAAGCTAAC	40.0	55.0	56.2	57.7	297 bp	1.0	63.6