Hotspot

trnT(UGU)-psbM

LSC · 23592-24635 · 1044 bp

Back to Cyananthus

Pi 0.0216
MarkerSeek score 71.00
Primer available yes
Label trnT(UGU)-psbM

Feature Metadata

label_name
trnT(UGU)-psbM
feature_type
igs
region
LSC
length_bp
1044
variable_sites
22
pi
0.0216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(UGU)-psbM_p1 AAGCCTACTCTCGGAATCGA AAAATGAACAGTGCCGTAGC 50.0 45.0 57.9 57.0 1156-1165 bp 1.0 75.0
trnT(UGU)-psbM_p2 AGCCTACTCTCGGAATCGAA AAAATGAACAGTGCCGTAGC 50.0 45.0 57.9 57.0 1155-1164 bp 1.0 75.0
trnT(UGU)-psbM_p3 CGATGACCTCCGCATTACAA AAAATGAACAGTGCCGTAGC 50.0 45.0 58.1 57.0 1134-1143 bp 1.0 74.9
trnT(UGU)-psbM_p4 GCCTACTCTCGGAATCGAAC AAAATGAACAGTGCCGTAGC 55.0 45.0 57.9 57.0 1154-1163 bp 1.0 74.4
trnT(UGU)-psbM_p5 GAATCGAACCGATGACCTCC AAAATGAACAGTGCCGTAGC 55.0 45.0 58.1 57.0 1143-1152 bp 1.0 74.3