Hotspot

rps16-trnD(GUC)

LSC · 5110-6060 · 951 bp

Back to Cyananthus

Pi 0.0106

MarkerSeek score 65.50

Primer available yes

Label rps16-trnD(GUC)

Feature Metadata

label_name: rps16-trnD(GUC)
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 10
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnD(GUC)_p1	TTCCTTCTCTTCGGGATCGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.9	58.5	1099-1106 bp	1.0	76.3
rps16-trnD(GUC)_p2	CGGTGCTTTTTACCACATCG	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.8	58.5	1049-1056 bp	1.0	75.5
rps16-trnD(GUC)_p3	CGGGATCGAACATCAATTGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.6	58.5	1088-1095 bp	1.0	74.3
rps16-trnD(GUC)_p4	ATAAGTCGCACGGTGCTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	1059-1066 bp	1.0	73.6
rps16-trnD(GUC)_p5	CTTCCTTCTCTTCGGGATCG	TTCAATTGGTCAGAGCACCG	55.0	50.0	57.5	58.5	1100-1107 bp	1.0	73.4