Hotspot

matK-trnK(UUU)

LSC · 3547-4274 · 728 bp

Back to Cyananthus

Pi 0.00824

MarkerSeek score 61.60

Primer available yes

Label matK-trnK(UUU)

Feature Metadata

label_name: matK-trnK(UUU)
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 6
pi: 0.00824
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK(UUU)_p1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.2	58.2	859 bp	1.0	73.0
matK-trnK(UUU)_p2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	858 bp	1.0	73.0
matK-trnK(UUU)_p3	CCATGATCATGAGCAAGTGC	GGGTTTGATTGCCTCGTCTA	50.0	50.0	57.2	57.6	941 bp	1.0	71.1
matK-trnK(UUU)_p4	CCATGATCATGAGCAAGTGC	TAGGGTTTGATTGCCTCGTC	50.0	50.0	57.2	57.6	943 bp	1.0	71.1
matK-trnK(UUU)_p5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.2	57.5	872 bp	1.0	70.7