Hotspot

rps2

LSC · 7049-7759 · 711 bp

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Pi 0.0901

MarkerSeek score 81.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 274
pi: 0.0901
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	CCCAAGACGCCATAAGTACC	TTGGCTAAGTCCTGTCAGGA	55.0	50.0	58.1	58.0	1523 bp	0.105263	43.6
rps2_p2	TCGACACTCGCTAGCTCATA	TTGGCTAAGTCCTGTCAGGA	50.0	50.0	58.1	58.0	1452 bp	0.105263	43.6
rps2_p3	AGGGACCATAACCTTCACCA	TTGGCTAAGTCCTGTCAGGA	50.0	50.0	57.9	58.0	1670 bp	0.105263	43.6
rps2_p4	AAAGGAACCCAGGGACCATA	TTGGCTAAGTCCTGTCAGGA	50.0	50.0	57.9	58.0	1680 bp	0.105263	43.5
rps2_p5	AAGGAACCCAGGGACCATAA	TTGGCTAAGTCCTGTCAGGA	50.0	50.0	57.9	58.0	1679 bp	0.105263	43.5