Hotspot

rbcL

LSC · 25464-26930 · 1467 bp

Back to Cuscuta

Pi 0.066

MarkerSeek score 80.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 416
pi: 0.066
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ACCGACCGGAACACTTATTG	GTGCTGAAAGTAGAAGCCCA	50.0	50.0	57.9	57.8	2500-2805 bp	0.210526	57.7
rbcL_p2	CGTCCTTGAACTACCAGAGC	ACACATTAGCCTGCAAGCAT	55.0	45.0	58.0	58.2	2468-2512 bp	0.157895	48.8
rbcL_p3	CGTCCTTGAACTACCAGAGC	GTGCTGAAAGTAGAAGCCCA	55.0	50.0	58.0	57.8	2609-2657 bp	0.157895	48.8
rbcL_p4	ACCGACCGGAACACTTATTG	ACACATTAGCCTGCAAGCAT	50.0	45.0	57.9	58.2	2616-2660 bp	0.157895	47.9
rbcL_p5	CACCGACCGGAACACTTATT	ACACATTAGCCTGCAAGCAT	50.0	45.0	57.9	58.2	2617-2661 bp	0.157895	47.9