Hotspot

psbD

LSC · 11437-12498 · 1062 bp

Back to Cuscuta

Pi 0.0554

MarkerSeek score 80.90

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 254
pi: 0.0554
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	GCGGTAGAGTAACACCATGG	GACAATCCATCCGTCTCCAC	55.0	55.0	58.1	58.1	2235-2457 bp	0.578947	74.2
psbD_p2	GCGGTAGAGTAACACCATGG	CTAGAGTCGCTAAGTGGGGA	55.0	55.0	58.1	57.9	1846-2023 bp	0.368421	65.8
psbD_p3	GCGGTAGAGTAACACCATGG	CACCCCCAAAATAAAGGGCT	55.0	50.0	58.1	58.0	2290 bp	0.105263	43.3
psbD_p4	TCAGCGGTAGAGTAACACCA	CACCCCCAAAATAAAGGGCT	50.0	50.0	58.1	58.0	2293 bp	0.105263	43.1
psbD_p5	GCGTCAAGCGACTATTCTCA	CACCCCCAAAATAAAGGGCT	50.0	50.0	58.1	58.0	2034 bp	0.105263	43.1