Hotspot

psbC

LSC · 12446-13867 · 1422 bp

Back to Cuscuta

Pi 0.0673

MarkerSeek score 80.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 373
pi: 0.0673
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCCTTTAACCCAACGCAATC	AATCGAACCCGCATCTTCTC	50.0	50.0	58.0	58.1	2441-2553 bp	0.578947	74.6
psbC_p2	GCCTTTAACCCAACGCAATC	GAATCGAACCCGCATCTTCT	50.0	50.0	58.0	58.1	2442-2554 bp	0.578947	74.6
psbC_p3	GATGGGAGTTTCCGGTGTAC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	1465-2658 bp	0.578947	74.5
psbC_p4	TTTCCCAAGAAATTCGGGCA	AATCGAACCCGCATCTTCTC	45.0	50.0	58.0	58.1	2249-2357 bp	0.263158	62.0
psbC_p5	TTTCCCAAGAAATTCGGGCA	GAATCGAACCCGCATCTTCT	45.0	50.0	58.0	58.1	2250-2358 bp	0.263158	62.0