Hotspot

rpl20-rps12

LSC · 36299-37044 · 746 bp

Back to Cuscuta

Pi 0.143

MarkerSeek score 80.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 370
pi: 0.143
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCACAAACGCCGAAAATCTC	TGTCCCCAACAGAAGCAAAA	50.0	45.0	58.0	57.8	1172-1189 bp	0.157895	48.5
rpl20-rps12_p2	CCACAAACGCCGAAAATCTC	TTGTCCCCAACAGAAGCAAA	50.0	45.0	58.0	57.8	1173-1190 bp	0.157895	48.5
rpl20-rps12_p3	CCACAAACGCCGAAAATCTC	ATTTGTCAGCCGTATGCAGT	50.0	45.0	58.0	57.9	1449-1466 bp	0.157895	48.4
rpl20-rps12_p4	AGACGAGTGATCCACAAACG	ATTTGTCAGCCGTATGCAGT	50.0	45.0	57.9	57.9	1460-1477 bp	0.157895	48.1
rpl20-rps12_p5	GCCCGTATTCGAGAAGCTAG	ATTTGTCAGCCGTATGCAGT	55.0	45.0	57.9	57.9	1893 bp	0.105263	42.9