Hotspot

trnT-GGU-psbD

LSC · 10767-11436 · 670 bp

Back to Cuscuta

Pi 0.258

MarkerSeek score 77.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 490
pi: 0.258
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTAACACCATGGTAAGGCGT	TTGACCAAGGGTTATAGTCA	50.0	40.0	57.9	53.2	553-786 bp	0.789474	44.7
trnT-GGU-psbD_p2	GTAACACCATGGTAAGGCGT	TGACCAAGGGTTATAGTCAT	50.0	40.0	57.9	53.0	552-785 bp	0.789474	43.6
trnT-GGU-psbD_p3	GTAACACCATGGTAAGGCGT	GATTGACCAAGGGTTATAGTCA	50.0	40.9	57.9	55.2	555-743 bp	0.736842	42.6
trnT-GGU-psbD_p4	ACACCATGGTAAGGCGTAAG	GATTGACCAAGGGTTATAGTCA	50.0	40.9	57.6	55.2	552-740 bp	0.684211	39.4
trnT-GGU-psbD_p5	CACCATGGTAAGGCGTAAGT	GATTGACCAAGGGTTATAGTCA	50.0	40.9	57.6	55.2	551-739 bp	0.684211	39.4