Hotspot

rps2-trnC-GCA

LSC · 7760-8987 · 1228 bp

Back to Cuscuta

Pi 0.273

MarkerSeek score 73.20

Primer available yes

Label rps2-trnC-GCA

Feature Metadata

label_name: rps2-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 827
pi: 0.273
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-trnC-GCA_p1	CTTGTTTCTGTGGTGGACCA	GCAGCACTTCTAGAGTCCAC	50.0	55.0	57.9	58.0	1348-2185 bp	0.578947	74.7
rps2-trnC-GCA_p2	CCCTGACAAATCGCGAAAAC	GCAGCACTTCTAGAGTCCAC	50.0	55.0	58.0	58.0	2434-2468 bp	0.157895	51.7
rps2-trnC-GCA_p3	CCTGACAAATCGCGAAAACC	GCAGCACTTCTAGAGTCCAC	50.0	55.0	58.0	58.0	2433-2467 bp	0.157895	51.7
rps2-trnC-GCA_p4	CCCTGACAAATCGCGAAAAC	AGAATTGATCAGGCGACACC	50.0	50.0	58.0	58.0	1993 bp	0.105263	43.7
rps2-trnC-GCA_p5	CCTGACAAATCGCGAAAACC	AGAATTGATCAGGCGACACC	50.0	50.0	58.0	58.0	1992 bp	0.105263	43.7