Hotspot

rpl22

LSC · 86580-86969 · 390 bp

Back to Curcuma

Pi 0.004

MarkerSeek score 39.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 390
variable_sites: 11
pi: 0.004
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	AGTGTTTATCATTCATTGATAGGAGA	47.6	30.8	58.1	56.3	528-535 bp	0.857143	34.4
rpl22_p2	ACCAAGTCTGAAACCAAGTGG	AGTGTTTATCATTCATTGATAGGAGA	47.6	30.8	58.3	56.3	529-536 bp	0.857143	34.4
rpl22_p3	CCAAGTCTGAAACCAAGTGGA	GTGTTTATCATTCATTGATAGGAGA	47.6	32.0	58.1	55.0	527-534 bp	0.857143	34.4
rpl22_p4	CCAAGTCTGAAACCAAGTGGA	AGTGTTTATCATTCATTGATAGGAG	47.6	32.0	58.1	55.0	528-535 bp	0.857143	34.4
rpl22_p5	CCAAGTCTGAAACCAAGTGG	AGTGTTTATCATTCATTGATAGGAGA	50.0	30.8	56.5	56.3	528-535 bp	0.857143	34.4