Hotspot

ccsA

SSC · 120873-121862 · 990 bp

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Pi 0.00245

MarkerSeek score 40.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 990
variable_sites: 18
pi: 0.00245
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATCCTATCCTATCA	TGGGTATGGGATGATGTTTTGT	55.0	40.9	58.8	57.7	1160 bp	1.0	56.4
ccsA_p2	TGGCGGCATCCTATCCTATC	TGGGTATGGGATGATGTTTTGT	55.0	40.9	58.8	57.7	1161 bp	1.0	56.4
ccsA_p3	GCGGCATCCTATCCTATCAA	TGGGTATGGGATGATGTTTTGT	50.0	40.9	56.9	57.7	1159 bp	1.0	54.4
ccsA_p4	GTGGCGGCATCCTATCCTAT	TGGGTATGGGATGATGTTTTGT	55.0	40.9	59.1	57.7	1162 bp	1.0	54.1
ccsA_p5	GCGGCATCCTATCCTATCAAA	TGGGTATGGGATGATGTTTTGT	47.6	40.9	57.7	57.7	1159 bp	1.0	52.2