Hotspot

atpF

LSC · 11100-12479 · 1380 bp

Back to Curcuma

Pi 0.00382

MarkerSeek score 38.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1380
variable_sites: 26
pi: 0.00382
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAATTCCGTCGCCTACTTGA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2083-2125 bp	1.0	83.1
atpF_p2	CAATTCCGTCGCCTACTTGA	TATTGCGAGACAACCGGAAG	50.0	50.0	58.0	58.0	2172-2214 bp	1.0	83.0
atpF_p3	CAATTCCGTCGCCTACTTGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	2273-2315 bp	1.0	82.5
atpF_p4	CCGTTTCGGCTACCACAATA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2650-2692 bp	1.0	82.4
atpF_p5	CCGTTTCGGCTACCACAATA	TATTGCGAGACAACCGGAAG	50.0	50.0	58.0	58.0	2739-2781 bp	1.0	82.3