Hotspot

ndhF-rpl32

SSC · 119007-119663 · 657 bp

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Pi 0.0103
MarkerSeek score 68.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
657
variable_sites
47
pi
0.0103
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGAATAAGTAGAAGCCCGA AGAAAAAGCCTTTACTGCAGC 50.0 42.9 56.8 57.3 840-909 bp 1.0 66.0
ndhF-rpl32_p2 CGGAATAAGTAGAAGCCCGA GAAAAAGCCTTTACTGCAGCT 50.0 42.9 56.8 57.3 839-908 bp 1.0 66.0
ndhF-rpl32_p3 TCGGAATAAGTAGAAGCCCG GAAAAAGCCTTTACTGCAGCT 50.0 42.9 56.8 57.3 840-909 bp 1.0 66.0
ndhF-rpl32_p4 TCGGAATAAGTAGAAGCCCG AGAAAAAGCCTTTACTGCAGC 50.0 42.9 56.8 57.3 841-910 bp 1.0 65.9
ndhF-rpl32_p5 GAAGCCCGAATCCTATTGACA GAAAAAGCCTTTACTGCAGCT 47.6 42.9 57.8 57.3 828-897 bp 1.0 65.9