Hotspot

rpl22-rps19

IRb · 86970-87143 · 174 bp

Back to Curcuma

Pi 0.0176

MarkerSeek score 57.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 174
variable_sites: 23
pi: 0.0176
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TCGCCCTTTGAAAACAGACA	ACGAGACATGCAAGAAACGA	45.0	45.0	58.0	57.9	283-299 bp	1.0	88.5
rpl22-rps19_p2	TCGCCCTTTGAAAACAGACA	TAACGGATCGTATGGTGGGT	45.0	50.0	58.0	58.2	339-355 bp	1.0	86.3
rpl22-rps19_p3	TCGCCCTTTGAAAACAGACA	GTGGGTCACAAATTGGGAGA	45.0	50.0	58.0	57.7	325-341 bp	1.0	86.2
rpl22-rps19_p4	TCGCCCTTTGAAAACAGACA	ATGGTGGGTCACAAATTGGG	45.0	50.0	58.0	58.4	328-344 bp	1.0	85.5
rpl22-rps19_p5	GCCCGCGAATTTGATCAATT	ACGAGACATGCAAGAAACGA	45.0	45.0	57.8	57.9	308-321 bp	0.952381	83.8