Hotspot

petD-rpoA

LSC · 80721-80914 · 194 bp

Back to Curcuma

Pi 0.014

MarkerSeek score 55.10

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 8
pi: 0.014
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CGTCGTCCAGTAGCTACAAC	TCAGACACTTTACGGAAGCA	55.0	45.0	58.1	56.8	363-380 bp	1.0	76.0
petD-rpoA_p2	TTTTTGATCGGTACCGCAGT	TCAGACACTTTACGGAAGCA	45.0	45.0	57.8	56.8	339-356 bp	1.0	75.6
petD-rpoA_p3	TCGTCGTCCAGTAGCTACAA	TCAGACACTTTACGGAAGCA	50.0	45.0	58.2	56.8	364-381 bp	1.0	75.3
petD-rpoA_p4	TTCGTCGTCCAGTAGCTACA	TCAGACACTTTACGGAAGCA	50.0	45.0	58.2	56.8	365-382 bp	1.0	75.3
petD-rpoA_p5	CATTTCGTCGTCCAGTAGCT	TCAGACACTTTACGGAAGCA	50.0	45.0	57.7	56.8	368-385 bp	1.0	74.6