Pi
0.00618
MarkerSeek score
61.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1097-1216 bp | 1.0 | 85.7 |
| trnT-UGU-trnL-UAA_p2 | GAGTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 1128-1247 bp | 1.0 | 85.4 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1105-1224 bp | 1.0 | 85.1 |
| trnT-UGU-trnL-UAA_p4 | GAGTCGAACCGATGACCATC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 1136-1255 bp | 1.0 | 84.9 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | ATCCAATCAAGTCCGTAGCG | 55.0 | 50.0 | 57.9 | 57.8 | 1120-1239 bp | 1.0 | 83.9 |