Hotspot

rps4-trnT-UGU

LSC · 47740-48200 · 461 bp

Back to Curcuma

Pi 0.0112

MarkerSeek score 60.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 23
pi: 0.0112
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTCCTGGTAAAGTCCCCAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	540-622 bp	1.0	83.3
rps4-trnT-UGU_p2	AGTCCTGGTAAAGTCCCCAG	GATGGTCATCGGTTCGACTC	55.0	55.0	58.3	58.2	509-591 bp	1.0	83.2
rps4-trnT-UGU_p3	GTCCCCAGACGACGTATTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.6	57.9	528-610 bp	1.0	83.1
rps4-trnT-UGU_p4	GTCCCCAGACGACGTATTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.6	58.2	497-579 bp	1.0	83.0
rps4-trnT-UGU_p5	AGTCCTGGTAAAGTCCCCAG	TATGTGATATGTGAGCCCGC	55.0	50.0	58.3	57.5	564-646 bp	1.0	80.6