Hotspot

trnS-UGA-psbZ

LSC · 37289-37646 · 358 bp

Back to Curcuma

Pi 0.00467

MarkerSeek score 48.70

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 358
variable_sites: 8
pi: 0.00467
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	AGCAAATACAACGGGTACACT	55.0	42.9	58.0	57.6	448-495 bp	1.0	73.0
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	AGCAAATACAACGGGTACACT	55.0	42.9	58.1	57.6	455-502 bp	1.0	72.7
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	AGCAAATACAACGGGTACACT	55.0	42.9	58.1	57.6	456-503 bp	1.0	72.7
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	AGCAAATACAACGGGTACACT	55.0	42.9	58.1	57.6	463-510 bp	1.0	72.6
trnS-UGA-psbZ_p5	CGGAGCTATCAACCACTCAG	AGCAAATACAACGGGTACACT	55.0	42.9	57.8	57.6	457-504 bp	1.0	71.4