Hotspot

petN-psbM

LSC · 29139-30495 · 1357 bp

Back to Curcuma

Pi 0.00724

MarkerSeek score 54.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1357
variable_sites: 45
pi: 0.00724
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1403-1474 bp	1.0	74.4
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1402-1473 bp	1.0	74.4
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1400-1471 bp	1.0	73.4
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1462-1533 bp	1.0	73.1
petN-psbM_p5	TGGGGAAGGAGTGGACTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1399-1470 bp	1.0	71.9