Hotspot

rpoB-trnC-GCA

LSC · 27052-28138 · 1087 bp

Back to Curcuma

Pi 0.00717

MarkerSeek score 57.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 38
pi: 0.00717
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTC	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.9	58.1	1197-1318 bp	1.0	85.1
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTC	GCGACACCCAGATTTGAACT	50.0	50.0	57.9	58.5	1223-1344 bp	1.0	81.8
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTC	CCTTACCACTTGGCCATGTC	50.0	55.0	57.9	58.5	1177-1298 bp	1.0	81.4
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.4	58.1	1198-1319 bp	1.0	81.3
rpoB-trnC-GCA_p5	CTTCCGTCAAGCCCTCATTA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.3	58.1	1193-1314 bp	1.0	80.5